MICROFLUIDIC-BASED PREPARATION OF HYALURONIC ACID MICROGELS FOR PROGESTERONE DELIVERY

Referencia Apresentador Autores
(Instituição)
Resumo
18-013
Carlos Alberto Busatto Busatto, C.A.(Instituto de Desarrollo Tecnológico para la Industria Química); Casis, N.(Instituto de Desarrollo Tecnológico para la Industria Química); Luna, J.(Instituto de Desarrollo Tecnológico para la Industria Química); Ravaine, V.(Institut des Sciences Moléculaires); Estenoz, D.A.(Instituto de Desarrollo Tecnológico para la Industria Química (CONICET - UNL)); Labie, H.(Institut des Sciences Moléculaires); Lapeyre, V.(Institut des Sciences Moléculaires); Perro-Marre, A.(Institut des Sciences Moléculaires); Auzély-Velty, R.(Centre national de la recherche scientifique); Progesterone delivery systems had been proposed for regulating the estrous cycle in cattle by incorporating progesterone into biodegradable and non-biodegradable polymeric matrices. In recent years, polymer microgels have received considerable attention due to their great potential in the biomedical field as drug delivery systems. Hyaluronic acid (HA) is a naturally occurring glycosaminoglycan composed of N-acetyl-D-glucosamine and D-glucuronic acid. This polymer is abundant in nature, renewable, nontoxic, and can be chemically modified. In addition, HA can be cleaved by hyaluronidase present in vivo. The objective of this work is to preparate biodegradable HA microgels by a co-flow microfluidic strategy for the encapsulation and controlled release of progesterone. The approach relies on (i) the generation of a primary organic/water (O/W) nanoemulsion by the ultrasonication method, (ii) microfluidic emulsification of the primary emulsion, and (iii) photopolymerization of HA precursors with polymerizable methacrylate groups (HA-MA) present in the aqueous phase of the droplets, thereby transforming them into microgels. Their potential use for progesterone delivery applications was evaluated through degradability and encapsulation/release studies in PBS buffer at 37 °C in presence of different concentrations of hyaluronidase. These structures were enzymatically biodegradable and this property can be used to trigger the release of the hormone.
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