Evaluation of BMSCs response to PLA Scaffolds produced by FDM and Coating with Dopamine and Collagen

Referencia Apresentador Autores
Bruna Nunes Teixeira Teixeira, B.N.(Universidade Federal do Rio de Janeiro); Aprile, P.(Trinity College of Dublin); Kelly, D.J.(Trinity College of Dublin); Thiré, R.M.(Universidade Federal do Rio de Janeiro); Fused Deposition Modeling (FDM), a 3D printing technique, is an efficient method to produce porous scaffolds with complex geometries and different shapes. Polylactic Acid (PLA), a biodegradable and biocompatible polymer is currently applied to produced scaffolds for bone tissue engineering, however PLA is non-bioactive and it is necessary improve its surface. A mussel-inspired DOPA (Dopamine - DA) is proposed to coat different surfaces by spontaneous deposition of Polydopamine (PDA) and it has potential to enhance biological response of cells and to enable covalent immobilization of biomolecules. The aim of this work was to evaluate BMSCs viability, adhesion and metabolic activity in response to PLA scaffolds produced by FDM and functionalized with PDA and Collagen (COL). PLA scaffolds were printed with 1mm of strut spacing and coated with DA and collagen. Physical and chemical properties of scaffolds were characterized by EDS, dimensional deviation, porosity estimative by Archimedes principle and compression test. Surface composition was characterized by XPS. Cells that were cultivated in PLA scaffolds, coated, or not, with PDA, PDA/COL or COL were viable in day 0 and day 7. Nevertheless, the amount of cells was drastically raised to PLA/PDA/COL in comparison with PLA. After 24 hours, was possible to see that cells cultivated in scaffolds with different coatings were more spread and were expressing more vinculin, mainly for the group PLA/PDA/COL. The metabolic activity of BMSCs was significantly higher to PLA/PDA/COL scaffolds in comparison to the other groups. This study suggest that the coating with PDA can improve the cellular response to PLA scaffolds and work, efficiently and in an easy way, as a platform to covalent immobilization of collagen onto PLA scaffolds surface.
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